Patrick Adlercreutz - Forskningsoutput - Lunds universitet

biotransformation — Engelska översättning - TechDico

It catalyzes the decomposition of hydrogen peroxide to water and oxygen. The assay accurately demonstrates the characteristics of the cGAS enzyme. Double-stranded DNA activates the enzyme which generates cGAMP in the presence of both GTP and ATP. A 45 base pair ISD (interferon stimulatory DNA) and a 60 base pair HSV (herpes simplex virus) DNA both activate the enzyme. The microhemagglutination assay was replaced by the more sensitive and reproducible ELISA system, which has been applied in various formats including the immobilized antigen (direct) assay , an immobilized anti-PA antibody (antigen-capture) assay , and a competition ELISA , with, where reported, varying degrees of specificity and sensitivity (17,18,28). The enzyme appears in viruses and cellular organisms Synonyms cyclodextrin glycosyltransferase, cyclodextrin glucanotransferase, cyclomaltodextrin glucanotransferase, alpha-cgtase, beta-cgtase, toruzyme, alpha-cyclodextrin glycosyltransferase, cyclodextrin glucosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrin glycosyl transferase, more Catalase Enzyme Activity Assay Kit. NWLSS | Northwest Life Science Specialties, LLC. This assay is recommended for researchers wishing to assay catalase enzyme activity in tissue homogenates, cell lysates & other biological fluids or extracts where catalase is present.

CGTase activity assay. All enzyme assays were performed by incubating 0.1 ml of diluted enzyme (0.35 to 1.0 U/ml) with 2 ml of 2% (wt/vol) soluble starch in 50 mM citric acid/disodium hydrogen phosphate buffer (pH 5.5) at 45°C for 10 min. Enzyme assays The various reactions catalyzed by B. agaradhaerens LS-3C CGTase were measured by incubating the enzyme at 50 °C with substrate solutions in 50 mM Tris–HCl buffer, pH 8.0. In all cases, the substrate was preincubated at the same temperature for about 5 min prior to addition of the enzyme. One unit of CGTase is defined as the amount of enzyme catalyzing the production of 1 μmol of β-CD/min under the reaction conditions.2.4. Determination of CD concentrationThe α-CD concentration was assayed by the decrease in absorbance at 507 nm caused by a methyl orange α-CD complex formation . CGTase enzyme production levels.

Patrick Adlercreutz - Forskningsoutput - Lunds universitet

The reaction mixture containing 40 mg of soluble starch in 1.0 mL of 0.1 M phosphate buVer (pH 6.0) and 0.1 mL of enzyme solution was incubated at 60°C for 10 min. Cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) is a unique member of glycoside hydrolase family 13. Its defining property is the ability to form cyclodextrins from starch, through an intramolecular transglycosylation reaction (cyclization).

Patrick Adlercreutz - Forskningsoutput - Lunds universitet

Enzyme assay of N-acetylglucosaminyltransferase III (GnT-III) Authors Korekane, Hiroaki Department of Disease Glycomics (Seikagaku Corporation), The Institute of Scientific and Industrial Research, Osaka University Roodyn DB. A generalized system for automation of enzyme assays. Biochem J. 1970 Oct; 119 (5):823–837. [PMC free article] SCHAFFERT RR, KINGSLEY GR, GETCHELL G. AUTOMATED DETERMINATION OF SERUM GLUTAMIC OXALACETIC AND GLUTAMIC PYRUVIC TRANSAMINASE. Clin Chem. 1964 Jun; 10:519–532. 12 Sep 2002 The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes several carbon, nitrogen and mineral sources, were assayed. 31 Aug 2018 CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are Assay for cyclization activity of α-CGTase.

One unit of enzyme activity was de¢ned as the amount of enzyme that forms 1 Wmol of Q-CD from soluble starch in 1 min. Alkaline phos-phatase was assayed by the method of Yamane et al.
Kakkirurgi karlstad

Clin Chem. 1964 Jun; 10:519–532. 12 Sep 2002 The extracellular enzyme cyclodextrin glucanotransferase (CGTase) synthesizes several carbon, nitrogen and mineral sources, were assayed. 31 Aug 2018 CGTase is an industrially important enzyme for α-, β- or γ-cyclodextrins (CDs) production, which are Assay for cyclization activity of α-CGTase. Therefore, CGTase G1 could potentially serve as an industrial enzyme for the The assay was conducted by reacting 200 μL CGTase sample (or γ-CD 2.3 Assay of enzyme activity.

Here is an example for a 1 mL enzyme assay reaction that needs Zn+2 ion.
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biotransformation — Engelska översättning - TechDico

CGTase enzyme activity assay CGTase activity was measured by PHP method described by Goel and Nene (1995). Briefly, 1mL cell-free supernatant of 24 h old bacterial culture grown in Horikoshi –II medium was added to 1 mL 1% starch solution prepared in 50 mM 2017-5-3 · used to enhance the production of CGTase (6, 8 & 11). This study presents the screening of Bacillus flexus MSBC 2, which is a potent producer of CGTase and the optimization of cultural conditions for maximum enzyme production. Materials and Methods Isolation screening and identification of β- 2013-7-15 · Recombinant CGTase was expressed at different temperature for the corresponding post-induction time. 2.3. CGTase enzyme assay Enzyme assay was carried out according to the Kaneko method [10]. The reaction mixture containing 1ml of 40mg of soluble starch in 0.1M potassium phosphate buffer (pH 6.0) and 0.1ml of the crude enzyme from the culture, and CGTase Enzyme Production 2.5.