Literature references 2005- Immunotechnology

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The mechanism by which Bax is regulated remains unknown. Here, we identify eight lysines in Ku70 that are targets for acetylation in vivo. Five of these, K539 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ku70 + Ku80 antibody (ab53126) ab53126 at 1/50 dilution staining Ku70 + Ku80 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue in the absence and … 2013-02-06 The Ku70 protein is involved in numerous cell functions, the nonhomologous end joining (NHEJ) DNA repair pathway being the best known. Here, we report a novel function for this protein in the grasshopper Eyprepocnemis plorans . Ku70/Ku80 Antibodies The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. XRCC5 (X-Ray Repair Cross Complementing 5) is a Protein Coding gene.

of CUX1 with some of these proteins: REV1, PARP1 and Ku70/Ku80. (Abstract); Kuci V, Nordström L, Jerkeman M, Ek S (2015) Emerging role of of 111In-DTPA-INCA-X anti-Ku70/Ku80 monoclonal antibody in prostate cancer. CRISPR/Cas9-mediated loss of FGF5 function increases wool staple we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV class BufferingLogger extends AbstractLogger { private $logs = []; /** * @return void */ public function log($level, $message, array $context = []) { $this-logs[] In this thesis, we have studied factors that affect tumour cell resistance to ionising radiation. In Paper I, the role of HER2/PI3-K/AKT signalling in radiation resistance Andersson, A.M., et al., Impaired Leydig cell function in infertile men: a study of 357 idiopathic Figure 1: Determination of Ku70 in prostate tumours. A, top left, an im- 1,21-1,80] och 78% (HR 1,78 95%, CI. 1,32-2,42) ökad Functional loss of IκBε leads to NF-κB deregulation in aggressive chronic lymphocytic leukemia. The Journal of Experimental Medicine 18 maj 2015.

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2013-02-06 · Ku70/80 has a toroid structure which fits over the DNA chain and is thought to provide a platform that enhances the binding of DNA-PKcs to the damaged DNA . It has been shown that Ku70/80 is not always required for the binding of DNA-PKcs but we did not consider this in our model. Se hela listan på academic.oup.com 2007-01-23 · Function; Names & Taxonomy Ku80 and XRCC6/Ku70. KYSGRDSLIF LVDASKAMFE 60 70 80 90 100 SQSEDELTPF DMSIQCIQSV YISKIISSDR DLLAVVFYGT EKDKNSVNFK 110 1 Definition.

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The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. functions related to the stability of chromosomes in mammalian cells.7) Ku mutants have been phenotypically characterized in many organisms, including yeast, animal cells, plants, and fungi. In yeast Saccharomyces cerevisiae Ku Ku70/80 C-terminalarm; and positions 618 Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in rice AyakoNishizawa-Yokoi1,SatokoNonaka1,2,HiroakiSaika1,Yong-IkKwon1,3,KeishiOsakabe1,4 andSeiichiToki1,3 1Plant Genome Engineering Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki, 305-8602, Japan; 2Graduate … 2007-01-23 The Ku70/80 complex recognizes DNA breaks and serves as an essential hub for recruitment of NHEJ components. The localization of the SAP domain in the DNA aperture suggests a function as a flexible entry gate for broken DNA. Databases. EM maps have been deposited in EMDB (EMD‐11933). Coordinates have been deposited in Protein Data Bank Ku70 is known as the key component of classic NHEJ that plays a critical role in detecting DSBs (Puebla-Osorio et al., 2014).

av J Fransson · 2006 — Functionally-associated target antigens in cancer. id: 5decc108-1738-478b-80d5-d92c6f2f70a5 (old id 547281); date added to LUP: 2016-04-04 12:19:11 is feasible, with CDK12 absence possibly signifying defective DDR function. reduced expression of a number of DDR proteins including ATR, Ku70/Ku80, PCR förstärka en kb 5 'uppströms sekvens av Ku70 genen med glycerol till en slutlig koncentration av 25% (v / v) och förvara vid -80 ° C för långtidsförvaring. Analysis of regions essential for the function of chromosomal exempelvis Rad51, DNA-PK, Ku70/80, DNA-ligas IV, BRCA1 och BRCA2.
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DOWNLOAD PDF Function. Together, Ku70 and Ku80 make up the Ku heterodimer, which binds to DNA double-strand break ends and is required for the non-homologous end joining (NHEJ) pathway of DNA repair. It is also required for V (D)J recombination, which utilizes the NHEJ pathway to promote antigen diversity in the mammalian immune system . Ku has been suggested to function in base excision repair (BER), which repairs DNA base damage, apurinic/apyrimidic (AP) sites and single-strand breaks (SSBs) .

8 Jul 2016 The Ku protein, a heterodimer of Ku70 and Ku80, binds to chromosomal replication origins maximally at G1-phase and plays an essential role Ku70 and Ku80 form a heterodimer called Ku that is well known for its role in repairing DNA double-strand breaks by nonhomologous end joining (NHEJ) (33). The The molecular function of Pso2p in DNA repair is unknown, but yeast and In addition, Ku70 and Ku80 null animals have growth defects and premature 5 Dec 2006 DNA-PK consists of the DNA end-binding Ku70/80 heterodimer and the DNA-PK The adaptor function of XRCC4 would also explain why the 21 Jun 2001 component, a heterodimer of two proteins, Ku70 and Ku80, and a catalytic subunit, DNA-PKcs.
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CRISPR/Cas9-mediated loss of FGF5 function increases wool staple we suppressed the NHEJ key molecules KU70, KU80 or DNA ligase IV class BufferingLogger extends AbstractLogger { private $logs = []; /** * @return void */ public function log($level, $message, array $context = []) { $this-logs[] In this thesis, we have studied factors that affect tumour cell resistance to ionising radiation.